Genome editing is fundamental technology in modern biotechnology. Recent advances in genome editing have provided us opportunities to explore genetic information of several organisms. Especially, genome editing in domestic animals has been expected to establish novel animal lines which have high productivity. In this regard, this special issue will consist of the papers related to recent progress on genome editing specifically in domestic animals including poultry, cattle and swine. The papers introduce unique genome editing strategy and current limitations in each species, and discuss future applications for advanced livestock industry. Journal of Animal Science and Biotechnology is a leading open access journal publishing innovative research across agriculture, dairy and animal science.